PubMed
Wilcox LJ et al., J Biol Chem, 2002 Sep 6;277(36):32466-72Wilcox LJ et al., J Biol Chem, 2002 Sep 6;277(36):32466-72
Indirect
Microarray analysis - wt vs TF mutant
Positive
W303-1A
Cells grown aerobically in YPD medium to exponential phase
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Positive
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
PubMed
Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77
Indirect
Microarray analysis - wt vs TF mutant
Negative
S288c
YPD medium; mid-log phase
PubMed
Gallo-Ebert C et al., J Biol Chem, 2013 Dec 6;288(49):35466-77Gallo-Ebert C et al., J Biol Chem, 2013 Dec 6;288(49):35466-77
Indirect
Northern blot - wt vs TF mutant
Positive
S288c
Cells were grown to exponential phase at 30 .C in synthetic medium. Cells with pRS-ERG25 plasmid that contained ( ) or lacked ( ) all three SRE/AR1 c elements.