S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Skn7p	YLR109W	PubMed Lee J et al., J Biol Chem, 1999 Feb 19;274(8):4537-44	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Oxidative stress - Yeast cells from overnight culture were diluted to an OD600 of 0.01 in SC medium and incubated with shaking at 30 °C until they reached an OD of 0.3. The cells were then aliquoted and incubated in the absence or in the presence of t-BOOH (0.2 mM) for 20 min.
		PubMed Lee J et al., J Biol Chem, 1999 Jun 4;274(23):16040-6	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	20 minutes exposure to 0.2 mM H2O2 (SC growth medium)
		PubMed Rep M et al., Mol Microbiol, 2001 Jun;40(5):1067-83	Indirect	Northern blot - wt vs TF mutant	Positive	CEN.PK2-1C	Oxidative stress; 0.1 mM H2O2 added to cells grown in YPD medium to mid-exponential phase
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase
		PubMed He XJ et al., Mol Microbiol, 2005 Dec;58(5):1454-67	N/A		N/A	S288c	N/A
		PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed Lenssen E et al., Eukaryot Cell, 2007 Dec;6(12):2251-9	Direct	ChIP	N/A	S288c	basal conditions (exponential growth in rich medium)
		PubMed Ni L et al., Genes Dev, 2009 Jun 1;23(11):1351-63	Direct	Chip-on-chip	N/A	S288c	SD medium + NaCl 0,6M 30'
		PubMed Kelley R et al., PLoS Genet, 2009 May;5(5):e1000488	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	YPD 30.C from 0.1 mM H2O2 to 0.4 mM H2O2
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed Weiner A et al., PLoS Biol, 2012;10(7):e1001369	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed Maity S et al., Cell Rep, 2016 Jul 19;16(3):851-65	Indirect	iTRAQ-MS - wt vs TF mutant	Negative	S288c	5 mM DTT
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C