S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Rtg3p	YCR005C	PubMed Jia Y et al., Mol Cell Biol, 1997 Mar;17(3):1110-7	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	logarithmic-phase cultures grown on YPR medium (YP + 2% raffinose)
		PubMed Liu Z et al., Mol Cell Biol, 1999 Oct;19(10):6720-8	Indirect	Northern blot - wt vs TF mutant	Positive	PSY142	rho0 cells cultivated in YP medium with 2% raffinose
		PubMed Epstein CB et al., Mol Biol Cell, 2001 Feb;12(2):297-308	Indirect	Microarray analysis - wt vs TF mutant	Positive	PSY142 (MATALFA leu2 lys2 ura3rho+)	Mitochondrial dysfunction; rho0 petites grown in YP raffinose (2%) medium to exponential phase
		PubMed Guaragnella N et al., J Biol Chem, 2003 Nov 14;278(46):45882-7	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	YPRaffinose medium
		PubMed Chen S et al., J Biol Chem, 2010 Apr 2;285(14):10397-407	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	SD medium early stationary phase
		PubMed Chen L et al., Yeast, 2010 Jun;27(6):345-59	Indirect	lacZ - wt vs TF mutant	Positive	BY4742	CIT2-lacZ reporter gene was transformed into a wt vs isogenic bHLH knock-out RO+ strains (DELTArtg3 with mitochondrial DNA). CIT2-lacZ expression was assayed from cultures grown in the absence (I-C-) or presence of inositol and choline (I+C+) containing either low-phosphate (Pi low) or high-phosphate (Pi high).
		PubMed Chen L et al., Yeast, 2010 Jun;27(6):345-59	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4742	CIT2-lacZ reporter gene was transformed into a wt vs isogenic bHLH knock-out RO+ strains (DELTArtg3 with mitochondrial DNA). CIT2-lacZ expression was assayed from cultures grown in the absence (I-C-) or presence of inositol and choline (I+C+) containing either low-phosphate (Pi low) or high-phosphate (Pi high).
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed Miceli MV et al., Front Genet, 2012;():Miceli MV et al., Front Genet, 2012;():	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed Ruiz-Roig C et al., Mol Biol Cell, 2012 Nov;23(21):4286-96	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	NaCl (osmotic stress)
		PubMed Barreto L et al., Environ Microbiol, 2012 Nov;14(11):3026-42	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Without KCl
		PubMed Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000	Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in anaerobic glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in ethanol limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
		PubMed Chow J et al., Genetics, 2019 Jul;212(3):667-690	Indirect	RT-PCR - wt vs TF mutant	Positive	PC538	YPD medium
		PubMed Chow J et al., Genetics, 2019 Jul;212(3):667-690	Indirect	RNA-seq - wt vs TF mutant	Positive	PC538	YEP-Gal solid medium for 24 hr
		PubMed Romero AM et al., Biochim Biophys Acta Gene Regul Mech, 2019 Sep;1862(9):194414	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	30.C in SC (+Fe) and SC with 100 microM BPS (-Fe) for 9 hours
		PubMed , , ;():, , ;():	Indirect	Western blot - wt vs TF mutant	Positive	W303-1B	YPR medium: 1% Yeast extract (DIFCO), 2% Bacto Peptone (DIFCO), 2% raffinose