Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Oaf1p
|
YDR256C
|
PubMed
Karpichev IV et al., Mol Cell Biol, 1997 Jan;17(1):69-80Karpichev IV et al., Mol Cell Biol, 1997 Jan;17(1):69-80 |
Indirect |
lacZ - wt vs TF mutant |
Negative |
S288c |
cells grown in YP Glycerol medium |
PubMed
Karpichev IV et al., Mol Cell Biol, 1998 Nov;18(11):6560-70Karpichev IV et al., Mol Cell Biol, 1998 Nov;18(11):6560-70 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
W3031A |
YP-Glycerol-Oleate |
PubMed
Baumgartner U et al., J Biol Chem, 1999 Aug 6;274(32):22208-16Baumgartner U et al., J Biol Chem, 1999 Aug 6;274(32):22208-16 |
Direct |
DNA footprinting |
N/A |
S288c |
Yeast cells were grown in YP medium containing 0.3% glucose to an A 600 of 0.4, shifted to YPO (oleate) medium, and incubated for 12 h. Cells were harvested, washed, and resuspended in YP medium |
PubMed
Rottensteiner H et al., Biochem J, 2002;():Rottensteiner H et al., Biochem J, 2002;(): |
Indirect |
lacZ - wt vs TF mutant |
Positive |
MF24-6x |
Cells grown in YPD (0.05% glucose) medium with 0.2% oleic acid for 18 h |
Indirect |
Western blot - wt vs TF mutant |
Positive |
BJ1991 |
Cells grown in fatty acid medium with 0.01% oleic acid or 0.001% lauric acid for 12 h |
PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104 |
Direct |
Chip-on-chip |
N/A |
S288c |
YPD medium; mid-log phase |
PubMed
Smith JJ et al., Mol Syst Biol, 2007;():Smith JJ et al., Mol Syst Biol, 2007;(): |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
BY4742 or BY4739 |
5-h induction in oleate |
PubMed
Karpichev IV et al., J Biol Chem, 2008 Apr 18;283(16):10264-75Karpichev IV et al., J Biol Chem, 2008 Apr 18;283(16):10264-75 |
Direct |
ChIP |
N/A |
S288c |
Exponential phase cells cultivated in YP growth medium containing 2% glycerol as carbon source |
PubMed
Ratnakumar S et al., J Biol Chem, 2010 Apr 2;285(14):10703-14Ratnakumar S et al., J Biol Chem, 2010 Apr 2;285(14):10703-14 |
Direct |
ChIP |
N/A |
S288c |
Glucose derepression; Exponential-phase Dpip2 cells cultivated for 1 h in synthetic growth medium containing 0.1% oleate + 3% glycerol |
PubMed
Parua PK et al., J Biol Chem, 2014 Dec 19;289(51):35542-60Parua PK et al., J Biol Chem, 2014 Dec 19;289(51):35542-60 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
YP medium + 0.1% OLEATE |
PubMed
Bergenholm D et al., mSystems, 2018 Jul-Aug;3(4):Bergenholm D et al., mSystems, 2018 Jul-Aug;3(4): |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Steady state aerobic chemostat cultivation at 30 .C in glucose-limited minimal medium containing vitamins and trace metals and maintained at pH 5.0 |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Steady state aerobic chemostat cultivation at 30 .C in ethanol-limited minimal medium containing vitamins and trace metals and maintained at pH 5.0 |
PubMed
Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000 |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in anaerobic glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in ethanol limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in nitrogen limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |