Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Mig1p
|
YIL162W
|
PubMed
Nehlin JO et al., Nucleic Acids Res, 1992 Oct 25;20(20):5271-8Nehlin JO et al., Nucleic Acids Res, 1992 Oct 25;20(20):5271-8 |
Indirect |
Northern blot - wt vs TF mutant |
Negative |
S288c |
Yeast cultures were grown in rich raffinose media (1% yeast extract, 2% peptone, 3% raffmose) and glucose was then added to a final concentration of 2 %; cells were harvested after 40 min |
PubMed
Nehlin JO et al., EMBO J, 1990;():Nehlin JO et al., EMBO J, 1990;(): |
Direct |
EMSA |
N/A |
S288c |
raffinose |
PubMed
Lundin M et al., Mol Cell Biol, 1994 Mar;14(3):1979-85Lundin M et al., Mol Cell Biol, 1994 Mar;14(3):1979-85 |
Direct |
EMSA |
N/A |
S288c |
in vitro |
PubMed
Hu Z et al., Curr Genet, 1995 Aug;28(3):258-66Hu Z et al., Curr Genet, 1995 Aug;28(3):258-66 |
Direct |
EMSA |
N/A |
S288c |
in vitro |
PubMed
Lutfiyya LL et al., Mol Cell Biol, 1996 Sep;16(9):4790-7Lutfiyya LL et al., Mol Cell Biol, 1996 Sep;16(9):4790-7 |
Indirect |
Invertase - wt vs TF mutant |
Negative |
S288c |
YPD medium; mid-log phase |
PubMed
Zhou H et al., BMC Genet, 2001;():Zhou H et al., BMC Genet, 2001;(): |
Indirect |
Northern blot - wt vs TF mutant |
Negative |
S288c |
growth in YPD medium to log phase (glucose repression conditions) |
PubMed
Harkness TA et al., Genetics, 2004 Oct;168(2):759-74Harkness TA et al., Genetics, 2004 Oct;168(2):759-74 |
Indirect |
Northern blot - wt vs TF overexpression |
Positive |
S288c |
SD medium |
PubMed
Moreno F et al., Biochem Soc Trans, 2005;():Moreno F et al., Biochem Soc Trans, 2005;(): |
Direct |
EMSA |
N/A |
S288c |
in vitro |
PubMed
Westergaard SL et al., Biotechnol Bioeng, 2007 Jan 1;96(1):134-45Westergaard SL et al., Biotechnol Bioeng, 2007 Jan 1;96(1):134-45 |
Indirect |
Microarray analysis - wt vs TF mutant |
Negative |
S288c |
Exponential phase cells cultivated in Verduyn growth medium with 40 g/L glucose |
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Negative |
N/A |
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h |
PubMed
Sarma NJ et al., Genetics, 2007 Mar;175(3):1127-35Sarma NJ et al., Genetics, 2007 Mar;175(3):1127-35 |
Indirect |
Enzyme activity - wt vs TF mutant |
Negative |
S288c |
SC medium; mid-log phase |
PubMed
Westholm JO et al., BMC Genomics, 2008;():Westholm JO et al., BMC Genomics, 2008;(): |
Indirect |
Microarray analysis - wt vs TF mutant |
Negative |
S288c |
Exponential phase cells growing in YPD (2% glucose) |
PubMed
Lorenz DR et al., Proc Natl Acad Sci U S A, 2009 Jan 27;106(4):1145-50Lorenz DR et al., Proc Natl Acad Sci U S A, 2009 Jan 27;106(4):1145-50 |
Indirect |
lacZ - wt vs TF mutant |
Negative |
S288c |
SC medium; mid-log phase |
PubMed
Vega M et al., J Biol Chem, 2016 Apr 1;291(14):7267-85Vega M et al., J Biol Chem, 2016 Apr 1;291(14):7267-85 |
Direct |
ChIP |
Negative |
S288c |
grown in YEPD until an OD600 of 0.8 was reached. Afterwards half of the culture was exposed to YEPE for 60 min |
PubMed
Xiao W et al., J Proteome Res, 2018 May 4;17(5):1812-1825Xiao W et al., J Proteome Res, 2018 May 4;17(5):1812-1825 |
Indirect |
SWATH- and DIA-based proteomic workflow |
Positive |
ScY01Â |
YPD at 40 and 30.C separately for 14 h |