S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Leu3p	YKR069W	PubMed Boer VM et al., FEMS Yeast Res, 2005 Jul;5(10):885-97	Indirect	Microarray analysis - wt vs TF mutant	Negative	CEN.PK540-3A	Ammonium-limitation: Defined synthetic medium (NH4)2SO4, 1.0 g/l;K2SO4, 5.3 g/l;KH2PO4, 3.0 g/l; MgSO4.7H2O, 0.5 g/l; vitamins and trace elements.
Leu3p	YKR069W	PubMed Tang L et al., BMC Genomics, 2006;():Tang L et al., BMC Genomics, 2006;():	Indirect	Microarray analysis - wt vs TF overexpression	Positive	BY4741	The cells were cultured in uracil dropout medium (YNB-AA (Sigma) 6.7 g/L, 0.77 g/L, Ura DO Supp., and 2% glucose supplemented with G418 at 200 mg/L) with shaking at 30.C. The cells were harvested in log-phase growth (A600 ~ 0.7).