PubMed
Tang L et al., BMC Genomics, 2006;():Tang L et al., BMC Genomics, 2006;():
Indirect
Microarray analysis - wt vs TF overexpression
Positive
BY4741
The cells were cultured in uracil dropout medium (YNB-AA (Sigma) 6.7 g/L, 0.77 g/L, Ura DO Supp., and 2% glucose supplemented with G418 at 200 mg/L) with shaking at 30.C. The cells were harvested in log-phase growth (A600 ~ 0.7).
PubMed
Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77
Indirect
Microarray analysis - wt vs TF mutant
Positive
S288c
YPD medium; mid-log phase
PubMed
Börlin CS et al., FEMS Microbiol Lett, 2020 Jul 1;367(13):Börlin CS et al., FEMS Microbiol Lett, 2020 Jul 1;367(13):
Direct
ChIP-seq
N/A
CEN.PK 113-5D
After 70 h of growth in minimal medium with 2% glucose, at 30.C and 200 rpm.