S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Ifh1p	YGL030W	PubMed Wade JT et al., Nature, 2004 Dec 23;432(7020):1054-8	Direct	Chip-on-chip	N/A	S288c	YPD
		PubMed Schawalder SB et al., Nature, 2004 Dec 23;432(7020):1058-61	Direct	ChIP	N/A	W303	Diauxic shift, YPAD (2% Bactopeptone, 1% yeast extract, 0.01% adenine, 2% Glucose).
			Indirect	RT-PCR - wt vs TF mutant	Positive	W303	YPAD medium (2% Bactopeptone, 1% yeast extract, 0.01% adenine, 2% Glucose), 30.C.
			Indirect	Microarray analysis - wt vs TF overexpression	Positive	W303	Cells were grown to 2 x107 cells/ml in YPLG medium (containing lactic acid and glycerol) and harvested 30 minutes after 2% galactose addition.
			Indirect	Microarray analysis - wt vs TF overexpression	Positive	W303	Cells were grown to 2 x107 cells/ml in YPLG medium (containing lactic acid and glycerol) and harvested 40 minutes after 2% galactose addition.
			Indirect	Microarray analysis - wt vs TF overexpression	Positive	W303	Cells were grown to 2 x107 cells/ml in YPLG medium (containing lactic acid and glycerol) and harvested 60 minutes after 2% galactose addition.
		PubMed Martin DE et al., Cell, 2004 Dec 29;119(7):969-79	Indirect	Northern blot - wt vs TF overexpression	Positive	S288c	SD medium to midlog phase and treated for 30 min with rapamycin
		PubMed Rudra D et al., EMBO J, 2005 Feb 9;24(3):533-42	Direct	ChIP	N/A	S288c	N/A
		PubMed Rudra D et al., Mol Cell Biol, 2007 Jul;27(13):4815-24	Direct	ChIP	N/A	S288c	N/A
		PubMed Lavoie H et al., PLoS Biol, 2010 Mar 9;8(3):e1000329	Direct	Chip-on-chip	N/A	S288c	YPD
		PubMed Downey M et al., Curr Biol, 2013 Sep 9;23(17):1638-48	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Cells expressing IFH1-13MYC-HIS3MX6 and IFH1-7K-R-13MYC-HIS3MX6 (all seven mapped lysine residues were mutated to arginine) were grown in YPAD overnight, and then diluted in YPLG (lactic acid and glycerol) medium to an OD600 of 0.1 and grown to an OD600 of 0.4. Glucose was then added to the culture to a 2% final concentration. Following glucose addition, total RNA was extracted at 0, 5, 10, 30 min
		PubMed Cai L et al., Cell Rep, 2013 Sep 26;4(6):1063-71	Direct	ChIP-seq	N/A	S288c	Library construction was performed using a protocol from the following website: http://bioinfo.mbb.yale.edu/array/Solexa_LibraryPrep_20080229ge.pdf Sequencing was performed on an Illumina GA IIX supervised by the UTSW Microarray Core Facility. Genome-wide occupancy profile of Ifh1-FLAG was obtained for cells collected from oxidative growth of the yeast metabolic cycle system
		PubMed Reja R et al., Genes Dev, 2015 Sep 15;29(18):1942-54	Direct	ChIP-exo	N/A	S288c	Strain was grown to OD600 0.8 at 25.C in 500 mL of YPD
		PubMed Kim MS et al., Biochim Biophys Acta, 2016 Aug;1859(8):1004-13	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Cells BY4741 ifh1 DELTA::loxP FHL1-3HA::kanMX6 p416-PIFH1-IFH1-13Myc-TADH1 and BY4741 ifh1 DELTA::loxP FHL1-3HA::kanMX6 p416-PIFH1-IFH1 T681A-13Myc-TADH1 were grown in YPD medium to exponential phases and treated with 200 ng/ml of rapamycin for 0, 10, 30 and 60 min
		PubMed Albert B et al., Mol Cell, 2016 Nov 17;64(4):720-733	Direct	ChIP	N/A	S288c	ifh1-6 allele cells with and without UTP22 depression (strain carrying UTP22-AID), treated with rapamycin for 60 min
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C