The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Hsf1p
|
YFL016C
|
PubMed
Tachibana T et al., J Biol Chem, 2002 Jun 21;277(25):22140-6Tachibana T et al., J Biol Chem, 2002 Jun 21;277(25):22140-6 |
Indirect |
lacZ - wt vs TF mutant |
Positive |
S288c |
Heat Shock 23ºC - 39ºC |
PubMed
Lee TI et al., Science, 2002 Oct 25;298(5594):799-804Lee TI et al., Science, 2002 Oct 25;298(5594):799-804 |
Direct |
Chip-on-chip |
N/A |
S288c |
YPD medium; mid-log phase |
PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104 |
Direct |
Chip-on-chip |
N/A |
S288c |
N/A |
PubMed
Yamamoto A et al., J Biol Chem, 2005 Mar 25;280(12):11911-9Yamamoto A et al., J Biol Chem, 2005 Mar 25;280(12):11911-9 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
S288c |
YPD medium 39ºC (heat shock) |
PubMed
Workman CT et al., Science, 2006 May 19;312(5776):1054-9Workman CT et al., Science, 2006 May 19;312(5776):1054-9 |
Direct |
Chip-on-chip |
N/A |
S288c |
N/A |
PubMed
Eastmond DL et al., J Biol Chem, 2006 Oct 27;281(43):32909-21Eastmond DL et al., J Biol Chem, 2006 Oct 27;281(43):32909-21 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
W303-1A |
Cells grown in YPAD at 30.C to mid-log phase (A600 of 0.5) submitted to heat-shock (37.C, 15 min) |
PubMed
SolÃs EJ et al., Mol Cell, 2016 Jul 7;63(1):60-71SolÃs EJ et al., Mol Cell, 2016 Jul 7;63(1):60-71 |
Indirect |
Net-seq |
Positive |
S288c |
basal conditions |
PubMed
de Jonge WJ et al., EMBO J, 2017 Feb 1;36(3):274-290de Jonge WJ et al., EMBO J, 2017 Feb 1;36(3):274-290 |
Direct |
ChIP |
N/A |
S288C |
SC medium |
Indirect |
RT-PCR - wt vs TF mutant |
Negative |
S288C |
SC medium |
PubMed
Pincus D et al., Mol Biol Cell, 2018 Dec 15;29(26):3168-3182Pincus D et al., Mol Biol Cell, 2018 Dec 15;29(26):3168-3182 |
Direct |
ChIP-seq |
N/A |
BY4741 |
YPDA (with 0.002% adenine), non-heat shock, 5´heat shock and 120´heat shock |
Direct |
ChIP-seq |
N/A |
BY4741 |
YPDA (with 0.002% adenine) Rapamycin (1 microM), non-heat shock |
Direct |
ChIP-seq |
N/A |
BY4741 |
YPDA (with 0.002% adenine), 5' heat shock |
Indirect |
NAC-seq (nascent mRNA-sequencing) |
Positive |
BY4741 |
YPDA (with 0.002% adenine) Rapamycin (1 microM), non-heat shock |
Indirect |
NAC-seq (nascent mRNA-sequencing) |
Positive |
BY4741 |
YPDA (with 0.002% adenine), 5' heat shock |
PubMed
Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314 |
Direct |
ChIP-exo |
N/A |
BY4741 |
YPD medium, 25.C |
|