The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Gln3p
|
YDL215C
|
PubMed
Coschigano PW et al., Mol Cell Biol, 1991 Feb;11(2):822-32Coschigano PW et al., Mol Cell Biol, 1991 Feb;11(2):822-32 |
Indirect |
Enzyme activity - wt vs TF mutant |
Positive |
S288c |
Glutamine - Yeast cells were grown with glutamine as a nitrogen source |
PubMed
Stanbrough M et al., Proc Natl Acad Sci U S A, 1995 Oct 10;92(21):9450-4Stanbrough M et al., Proc Natl Acad Sci U S A, 1995 Oct 10;92(21):9450-4 |
Indirect |
lacZ - wt vs TF mutant |
Positive |
S288c |
cells grown on glutamine as nitrogen source |
PubMed
Blinder D et al., J Bacteriol, 1995 Jul;177(14):4190-3Blinder D et al., J Bacteriol, 1995 Jul;177(14):4190-3 |
N/A |
|
N/A |
S288c |
N/A |
PubMed
Daugherty JR et al., J Bacteriol, 1993 Jan;175(1):64-73Daugherty JR et al., J Bacteriol, 1993 Jan;175(1):64-73 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
SIGMA1278b |
Wickerham's minimal medium + proline |
PubMed
Cunningham TS et al., J Bacteriol, 1996 Jun;178(12):3470-9Cunningham TS et al., J Bacteriol, 1996 Jun;178(12):3470-9 |
Direct |
EMSA |
N/A |
S288c |
in vitro |
PubMed
Shamji AF et al., Curr Biol, 2000 Dec 14-28;10(24):1574-81Shamji AF et al., Curr Biol, 2000 Dec 14-28;10(24):1574-81 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
S288c |
30 min 50 nM treatment with rapamycin in YPD |
PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104 |
Direct |
Chip-on-chip |
N/A |
S288c |
N/A |
PubMed
Hernández H et al., Biochem Biophys Res Commun, 2011 Jan 21;404(3):859-64Hernández H et al., Biochem Biophys Res Commun, 2011 Jan 21;404(3):859-64 |
Direct |
ChIP |
N/A |
S288c |
3-aminotriazole |
PubMed
Georis I et al., J Biol Chem, 2011 Dec 30;286(52):44897-912Georis I et al., J Biol Chem, 2011 Dec 30;286(52):44897-912 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
Methionine sulfoximine |
PubMed
Rai R et al., Genetics, 2015 Nov;201(3):989-1016Rai R et al., Genetics, 2015 Nov;201(3):989-1016 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
gln3DELTAgat1DELTA cells were grown in YNB-proline medium with Gln31 –730-Myc13 or with Gln3L332D,L336D,M340D,L343D-Myc13 |
PubMed
Ponomarova O et al., Cell Syst, 2017 Oct 25;5(4):345-357.e6Ponomarova O et al., Cell Syst, 2017 Oct 25;5(4):345-357.e6 |
Indirect |
RNA-seq - wt vs TF mutant |
Negative |
S90 |
wt and DELTAgln3 cells were grown in YPAD medium |
|