PubMed
Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82
Indirect
Microarray analysis - wt vs TF mutant
Negative
BY4741
YNB media
PubMed
Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911
Indirect
Microarray analysis - wt vs TF mutant
Positive
EG328-1A
RNA prepared from wt and DELTAgcn4 cells treated for 1 h with 10 mM 3-AT
Indirect
Microarray analysis - wt vs TF mutant
Positive
EG328-1A
RNA prepared from wt and DELTAgcn4 cells treated for 1 h with 200 nM Rapamycin
PubMed
Fendt SM et al., Mol Syst Biol, 2010;():Fendt SM et al., Mol Syst Biol, 2010;():
Indirect
Microarray analysis - wt vs TF mutant
Negative
S288c
YPD medium; mid-log phase
PubMed
Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311
Direct
ChIP-seq
N/A
BY4741
Cells cultured in synthetic complete medium lacking isoleucine and valine (SC-Ilv) to log-phase (OD600=0.6-0.8) supplemented with SM at 1 microg/mL for 25 min to induce Gcn4 synthesis