PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104
Direct
Chip-on-chip
N/A
S288c
SC medium; mid-log phase
PubMed
Ernst J et al., Mol Syst Biol, 2007;():Ernst J et al., Mol Syst Biol, 2007;():
Direct
Chip-on-chip
N/A
S288c
Methylmethane sulfonate stress
PubMed
Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82
Indirect
Microarray analysis - wt vs TF mutant
Positive
BY4741
YNB media
PubMed
Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911
Indirect
Microarray analysis - wt vs TF mutant
Positive
EG328-1A
RNA prepared from wt and DELTAgcn4 cells treated for 1 h with 10 mM 3-AT
PubMed
Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311
Direct
ChIP-seq
N/A
BY4741
Cells cultured in synthetic complete medium lacking isoleucine and valine (SC-Ilv) to log-phase (OD600=0.6-0.8) supplemented with SM at 1 microg/mL for 25 min to induce Gcn4 synthesis
PubMed
Walvekar AS et al., Mol Biol Cell, 2018 Oct 24;29(26):3183-3200Walvekar AS et al., Mol Biol Cell, 2018 Oct 24;29(26):3183-3200
Indirect
RNA-seq - wt vs TF mutant
Negative
CEN.PK
RM (1% yeast extract, 2% peptone, and 2% lactate) medium grown cells shifted to MM (0.17% yeast nitrogen base without amino acids, 0.5% ammonium sulfate, and 2% lactate) medium with methionine supplementation
PubMed
Srinivasan R et al., PLoS Genet, 2020 Dec;16(12):e1009252Srinivasan R et al., PLoS Genet, 2020 Dec;16(12):e1009252
Direct
ChIP
N/A
CEN.PK
Cells cultured for 1h in MM medium (Yeast Nitrogen Base with glucose as a carbon source) + Met (2mM methionine)