PubMed
Lee TI et al., Science, 2002 Oct 25;298(5594):799-804Lee TI et al., Science, 2002 Oct 25;298(5594):799-804
Direct
Chip-on-chip
N/A
S288c
YPD medium; mid-log phase
PubMed
Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311
Direct
ChIP-seq
N/A
BY4741
Cells cultured in synthetic complete medium lacking isoleucine and valine (SC-Ilv) to log-phase (OD600=0.6-0.8) supplemented with SM at 1 microg/mL for 25 min to induce Gcn4 synthesis
PubMed
Walvekar AS et al., Mol Biol Cell, 2018 Oct 24;29(26):3183-3200Walvekar AS et al., Mol Biol Cell, 2018 Oct 24;29(26):3183-3200
Indirect
RNA-seq - wt vs TF mutant
Negative
CEN.PK
RM (1% yeast extract, 2% peptone, and 2% lactate) medium grown cells shifted to MM (0.17% yeast nitrogen base without amino acids, 0.5% ammonium sulfate, and 2% lactate) medium with methionine supplementation