PubMed
Mascarenhas C et al., Mol Biol Cell, 2008 Jul;19(7):2995-3007Mascarenhas C et al., Mol Biol Cell, 2008 Jul;19(7):2995-3007
Indirect
Microarray analysis - wt vs TF mutant
Negative
S288c
minimalSDmedia+0.5mM H2O2 for 15min
Indirect
Microarray analysis - wt vs TF mutant
Positive
S288c
minimalSDmedia+3-aminotriazole
PubMed
Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82
Indirect
Microarray analysis - wt vs TF mutant
Negative
BY4741
YNB media
PubMed
Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311
Direct
ChIP-seq
N/A
BY4741
Cells cultured in synthetic complete medium lacking isoleucine and valine (SC-Ilv) to log-phase (OD600=0.6-0.8) supplemented with SM at 1 microg/mL for 25 min to induce Gcn4 synthesis