PubMed
Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911
Indirect
Microarray analysis - wt vs TF mutant
Positive
EG328-1A
RNA prepared from wt and DELTAgcn4 cells treated for 1 h with 10 mM 3-AT
Indirect
Microarray analysis - wt vs TF mutant
Positive
EG328-1A
RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
PubMed
Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311
Direct
ChIP-seq
N/A
BY4741
Cells cultured in synthetic complete medium lacking isoleucine and valine (SC-Ilv) to log-phase (OD600=0.6-0.8) supplemented with SM at 1 microg/mL for 25 min to induce Gcn4 synthesis
PubMed
Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314