S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gcn4p	YGL121C	PubMed Kleinschmidt M et al., Mol Genet Genomics, 2005 Jun;273(5):382-93	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	3-aminotriazole
		PubMed Workman CT et al., Science, 2006 May 19;312(5776):1054-9	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Mascarenhas C et al., Mol Biol Cell, 2008 Jul;19(7):2995-3007	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	minimalSDmedia+0.5mM H2O2 for 15min
			Indirect	Microarray analysis - wt vs TF mutant	Negative	S288c	minimalSDmedia+3-aminotriazole
		PubMed Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82	Indirect	Microarray analysis - wt vs TF mutant	Negative	BY4741	YNB media
		PubMed Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 10 mM 3-AT
		PubMed Fendt SM et al., Mol Syst Biol, 2010;():	Indirect	Microarray analysis - wt vs TF mutant	Negative	S288c	YPD medium; mid-log phase
		PubMed Walvekar AS et al., Mol Biol Cell, 2018 Oct 24;29(26):3183-3200	Indirect	RNA-seq - wt vs TF mutant	Positive	CEN.PK	RM (1% yeast extract, 2% peptone, and 2% lactate) medium grown cells shifted to MM (0.17% yeast nitrogen base without amino acids, 0.5% ammonium sulfate, and 2% lactate) medium with methionine supplementation
		PubMed Srinivasan R et al., PLoS Genet, 2020 Dec;16(12):e1009252	Indirect	RNA-seq - wt vs TF mutant	Positive	CEN.PK	Cells cultured for 1h in MM medium (Yeast Nitrogen Base with glucose as a carbon source) + Met (2mM methionine)