PubMed
Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82
Indirect
Microarray analysis - wt vs TF mutant
Positive
BY4741
YNB media
PubMed
Fendt SM et al., Mol Syst Biol, 2010;():Fendt SM et al., Mol Syst Biol, 2010;():
Indirect
Microarray analysis - wt vs TF mutant
Negative
S288c
YPD medium; mid-log phase
PubMed
Yadav KK et al., Curr Genet, 2016 Nov;62(4):841-851Yadav KK et al., Curr Genet, 2016 Nov;62(4):841-851
Direct
EMSA
Negative
S288c
Grown in YEPD or SD medium with YNB containing 2% glucose and kanamycin (50microg/mL)
PubMed
Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311
Direct
ChIP-seq
N/A
BY4741
Cells cultured in synthetic complete medium lacking isoleucine and valine (SC-Ilv) to log-phase (OD600=0.6-0.8) supplemented with SM at 1 microg/mL for 25 min to induce Gcn4 synthesis
PubMed
Srinivasan R et al., PLoS Genet, 2020 Dec;16(12):e1009252Srinivasan R et al., PLoS Genet, 2020 Dec;16(12):e1009252
Indirect
RNA-seq - wt vs TF mutant
Positive
CEN.PK
Cells cultured for 1h in MM medium (Yeast Nitrogen Base with glucose as a carbon source) + Met (2mM methionine)