PubMed
Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82
Indirect
Microarray analysis - wt vs TF mutant
Positive
BY4741
YNB media
PubMed
Mittal N et al., Nat Commun, 2017 Sep 6;8(1):457Mittal N et al., Nat Commun, 2017 Sep 6;8(1):457
Direct
ChIP-seq
N/A
BY4741
Cells grown on YPD and collected in mid-log phase at OD600 of 0.4-0.8
PubMed
Walvekar AS et al., Mol Biol Cell, 2018 Oct 24;29(26):3183-3200Walvekar AS et al., Mol Biol Cell, 2018 Oct 24;29(26):3183-3200
Indirect
RNA-seq - wt vs TF mutant
Positive
CEN.PK
RM (1% yeast extract, 2% peptone, and 2% lactate) medium grown cells shifted to MM (0.17% yeast nitrogen base without amino acids, 0.5% ammonium sulfate, and 2% lactate) medium with methionine supplementation