S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gcn4p	YCR021C	PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82	Indirect	Microarray analysis - wt vs TF mutant	Negative	BY4741	YNB media
		PubMed Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - wt vs TF mutant	Negative	EG328-1A	RNA prepared from wt and DELTAgcn4 cells treated for 1 h with 10 mM 3-AT
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn4 cells treated for 1 h with 200 nM Rapamycin
			Indirect	Microarray analysis - wt vs TF mutant	Negative	EG328-1A	RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 10 mM 3-AT
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
		PubMed Uluisik I et al., PLoS One, 2011;6(11):e27772	Indirect	Microarray analysis - wt vs TF mutant	Negative	BY4741	20 mM boric acid for 1h
		PubMed Walvekar AS et al., Mol Biol Cell, 2018 Oct 24;29(26):3183-3200	Indirect	RNA-seq - wt vs TF mutant	Positive	CEN.PK	RM (1% yeast extract, 2% peptone, and 2% lactate) medium grown cells shifted to MM (0.17% yeast nitrogen base without amino acids, 0.5% ammonium sulfate, and 2% lactate) medium with methionine supplementation