Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Gcn4p
|
YBR248C
|
PubMed
Kuenzler M et al., J Bacteriol, 1993 Sep;175(17):5548-58Kuenzler M et al., J Bacteriol, 1993 Sep;175(17):5548-58 |
Indirect |
lacZ - wt vs TF mutant |
Positive |
S288c |
Minimal medium; mid-log phase |
PubMed
Springer C et al., J Biol Chem, 1996;():Springer C et al., J Biol Chem, 1996;(): |
Direct |
EMSA |
N/A |
S288c |
in vitro |
PubMed
Natarajan K et al., Mol Cell Biol, 2001 Jul;21(13):4347-68Natarajan K et al., Mol Cell Biol, 2001 Jul;21(13):4347-68 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
S288c |
SC medium + 100mM 3-aminotriazole |
PubMed
Yin Z et al., Proteomics, 2004 Aug;4(8):2425-36Yin Z et al., Proteomics, 2004 Aug;4(8):2425-36 |
Indirect |
Proteomics - wt vs TF mutant |
Positive |
S288c |
SC-HIS medium + 3-aminotriazole |
PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104 |
Direct |
Chip-on-chip |
N/A |
S288c |
SC medium; mid-log phase |
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
N/A |
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h |
PubMed
Ernst J et al., Mol Syst Biol, 2007;():Ernst J et al., Mol Syst Biol, 2007;(): |
Direct |
Chip-on-chip |
N/A |
S288c |
Methylmethane sulfonate stress |
PubMed
Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
BY4741 |
YNB media |
PubMed
Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
EG328-1A |
RNA prepared from wt and DELTAgcn4 cells treated for 1 h with 10 mM 3-AT |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
EG328-1A |
RNA prepared from wt and DELTAgcn4 cells treated for 1 h with 200 nM Rapamycin |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
EG328-1A |
RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 10 mM 3-AT |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
EG328-1A |
RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 200 nM Rapamycin |
PubMed
Fendt SM et al., Mol Syst Biol, 2010;():Fendt SM et al., Mol Syst Biol, 2010;(): |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
S288c |
YPD medium; mid-log phase |
PubMed
Albrecht TR et al., Mol Cell Biol, 2012;():Albrecht TR et al., Mol Cell Biol, 2012;(): |
Direct |
ChIP |
N/A |
S288c |
YPD medium; mid-log phase |
PubMed
McIsaac RS et al., Nucleic Acids Res, 2013 Feb 1;41(4):e57McIsaac RS et al., Nucleic Acids Res, 2013 Feb 1;41(4):e57 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
S288c |
YPD medium; mid-log phase |
PubMed
Mittal N et al., Nat Commun, 2017 Sep 6;8(1):457Mittal N et al., Nat Commun, 2017 Sep 6;8(1):457 |
Direct |
ChIP-seq |
N/A |
BY4741 |
Cells grown on YPD and collected in mid-log phase at OD600 of 0.4-0.8 |
PubMed
Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311 |
Direct |
ChIP-seq |
N/A |
BY4741 |
Cells cultured in synthetic complete medium lacking isoleucine and valine (SC-Ilv) to log-phase (OD600=0.6-0.8) supplemented with SM at 1 microg/mL for 25 min to induce Gcn4 synthesis |
PubMed
Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000 |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in anaerobic glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in ethanol limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |
PubMed
Srinivasan R et al., PLoS Genet, 2020 Dec;16(12):e1009252Srinivasan R et al., PLoS Genet, 2020 Dec;16(12):e1009252 |
Indirect |
RNA-seq - wt vs TF mutant |
Positive |
CEN.PK |
Cells cultured for 1h in MM medium (Yeast Nitrogen Base with glucose as a carbon source) + Met (2mM methionine) |