PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Negative
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
PubMed
Mascarenhas C et al., Mol Biol Cell, 2008 Jul;19(7):2995-3007Mascarenhas C et al., Mol Biol Cell, 2008 Jul;19(7):2995-3007
Indirect
RT-PCR - wt vs TF mutant
Negative
S288c
minimal SDmedia
Indirect
RT-PCR - wt vs TF mutant
Negative
S288c
minimalSDmedia+0.5mM H2O2 for 15min
PubMed
Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82
Indirect
Microarray analysis - wt vs TF mutant
Negative
BY4741
YNB media
PubMed
Walvekar AS et al., Mol Biol Cell, 2018 Oct 24;29(26):3183-3200Walvekar AS et al., Mol Biol Cell, 2018 Oct 24;29(26):3183-3200
Indirect
RNA-seq - wt vs TF mutant
Positive
CEN.PK
RM (1% yeast extract, 2% peptone, and 2% lactate) medium grown cells shifted to MM (0.17% yeast nitrogen base without amino acids, 0.5% ammonium sulfate, and 2% lactate) medium with methionine supplementation