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Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription
Factor
Target
ORF/Genes
References Evidence
Code
Evidence
Experiment
Association
Type
Strain Environmental
Condition
Gat1p YHL016C PubMed external link Zhao X(1,) et al., Sci Rep, 2016;():Zhao X(1,) et al., Sci Rep, 2016;(): Indirect RT-PCR - wt vs TF mutant Positive S288c YNB medium, with glutamine or proline
PubMed external link Hong J et al., Elife, 2018 Apr 5;7(0):e32323Hong J et al., Elife, 2018 Apr 5;7(0):e32323 Indirect RNA-seq - wt vs TF mutant Negative FY4 Cells cultured in ammonium-limited chemostats
PubMed external link Zhang P et al., J Agric Food Chem, 2018 Oct 17;66(41):10881-10889Zhang P et al., J Agric Food Chem, 2018 Oct 17;66(41):10881-10889 Indirect RT-PCR - wt vs TF mutant Positive BY4741 DELTAgzf3 vs DELTAgzf3+overIndirect of GAT1 gene: strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
Indirect RT-PCR - wt vs TF mutant Positive BY4741 wt vs wt-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
Indirect RT-PCR - wt vs TF mutant Positive BY4741 wt vs DELTAgzf3-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
Indirect RT-PCR - wt vs TF mutant Negative BY4741 wt vs DELTAgln3-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
Indirect RT-PCR - wt vs TF mutant Positive BY4741 wt vs DELTAgat1-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
Indirect RT-PCR - wt vs TF mutant Negative BY4741 wt vs DELTAdal80-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
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