S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Cbf1p	YPR167C	PubMed Kuras L et al., FEBS Lett, 1995 Jun 19;367(1):15-8	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Yeast cells were grown in 150 ml of B medium (growth medium with no sulfur element) in the presence of a repressing amount (1 mM) of L-methionine as sulfur source. When the cells reached a density of about 107 cells per ml, they were harvested by filtration and washed with 150 ml of B medium. The cells were then suspended in 100 ml of B medium without methionine at 28°C
		PubMed O'Connell KF et al., Mol Cell Biol, 1995;():	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Yeast cells were pregrown in 1.0 mM methionine-containing medium before being shifted into medium lacking methionine or, as the control, back into fresh methionine-containing medium.
		PubMed Kuras L et al., EMBO J, 1997 May 1;16(9):2441-51	Direct	DNA footprinting	N/A	S288c	in vitro
		PubMed Aranda A et al., Appl Environ Microbiol, 2004 Apr;70(4):1913-22	Indirect	Western blot - wt vs TF mutant	Positive	S288c	1 g/L acetaldehyde (1H)
		PubMed Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	YNB media supplemented with 20 mg/L methionine
		PubMed Lavoie H et al., PLoS Biol, 2010 Mar 9;8(3):e1000329	Direct	Chip-on-chip	N/A	S288c	YPD
		PubMed Cormier L et al., Nucleic Acids Res, 2010 Aug;38(15):4998-5014	Direct	ChIP	N/A	W303	Isogenic strain expressing Myc-tagged version of Cbf1 was grown to early log phase in YPD medium and exposed to 0.5mM Cd2+.
		PubMed Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000	Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in anaerobic glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in ethanol limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in nitrogen limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h