S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Aft1p	YER145C	PubMed Yamaguchi-Iwai Y et al., EMBO J, 1996 Jul 1;15(13):3377-84	Indirect	Northern blot - wt vs TF overexpression	Positive	S288c	iron-repleted medium (100mM Ferric ammonium sulfate)
		PubMed Gross C et al., J Biol Chem, 2000 Oct 13;275(41):32310-6	Direct	DNA footprinting	Positive	S288c	low copper complete medium
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Courel M et al., Mol Cell Biol, 2005 Aug;25(15):6760-71	Indirect	Northern blot WT vs TF mutant	Positive	Y14438	Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C.
			Indirect	Northern blot WT vs TF mutant	Positive	Y14438	Iron- replete conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ) with 100uM ferric ammonium sulfate; 30.C.
			Indirect	Northern blot - wt vs TF mutant	Positive	SCMC01	Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C.
			Indirect	Northern blot - wt vs TF mutant	Positive	SCMC01	Iron- replete conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ) with 100uM ferric ammonium sulfate; 30.C.
			Indirect	Microarray analysis - wt vs TF mutant	Positive	SCMC01	Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C.
		PubMed Dubacq C et al., Mol Genet Genomics, 2006 Feb;275(2):114-24	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD
		PubMed Pagani MA et al., Mol Microbiol, 2007 Jul;65(2):521-37	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	aft1DELTA versus wt: Cells exposed to 5mM ZnCl2 for 30 minutes in YPD media
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed PÃ©rez-Sampietro M et al., Fungal Genet Biol, 2014;():	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	WT and DELTAaft1 cells growing exponentially in YPD liquid medium and then treated with 2 mM selenite for 3h or 6h. SNR19 was employed as loading control.
		PubMed Andreadis C et al., Nucleic Acids Res, 2014 Nov 10;42(20):12650-67	Direct	ChIP	N/A	S288c	Iron deprivation
		PubMed Caballero-Molada M et al., Biochem J, 2018 Apr 30;475(8):1523-1534	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	SD medium, mid exponential phase
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Cells cultured to mid-exponential phase in SD medium
		PubMed Jordá T et al., J Fungi (Basel), 2021 Oct 25;7(11):	Indirect	RT-PCR - wt vs TF overexpression	Positive	BY4741	SC medium
		PubMed Jordá T et al., J Fungi (Basel), 2021 Oct 25;7(11):	Indirect	RT-PCR - wt vs TF overexpression	Positive	BY4741	SC medium with 100 microM BPS for 9 h (Iron Deficiency)